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Title Apoptosis and cancer : methods and protocols / edited by Gil Mor and Ayesha B. Alvero
Published Totowa, N.J. : Humana Press, ©2008

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Description 1 online resource (xi, 266 pages) : illustrations
Series Methods in molecular biology ; v. 414
Methods in molecular biology (Clifton, N.J.) ; v. 414
Contents Modulation of apoptosis to reverse chemoresistance / Gil Mor, Michele K. Montagna, and Ayesha B. Alvero -- Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis / Suparna Mazumder, Dragos Plesca, and Alexandru Almasan -- Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate / David Diaz [and others] -- Detection of cancer-related proteins in fresh-frozen ovarian cancer samples using laser capture microdissection / Dan-Arin Silasi [and others] -- Flow cytometric detection of activated caspase-3 / Richard Fox and Martine Aubert -- The chemofx® assay : an ex vivo chemosensitivity and resistance assay for predicting patient response to cancer chemotherapy / Stacey L. Brower, Jeffrey E. Fensterer, and Jason E. Bush -- Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines / Ayesha B. Alvero, Michele K. Montagna, and Gil Mor -- Assessing expression of apoptotic markers using large cohort tissue microarrays / Elah Pick, Mary M. McCarthy, and Harriet M. Kluger -- Mitochondria potential, bax "activation," and programmed cell death / C. Michael Knudson and Nicholas M. Brown -- In vitro and in vivo apoptosis detection using membrane permeant fluorescent-labeled inhibitors of caspases / Brian W. Lee [and others] -- Caspase activity assays / Andrew L. Niles, Richard A. Moravec, and Terry L. Riss -- Multiplex caspase activity and cytotoxicity assays / Andrew L. Niles, Richard A. Moravec, and Terry L. Riss -- Homogeneous, bioluminescent proteasome assays / Martha A. O'Brien [and others] -- A simple method for profiling mirna expression / Jia-Wang Wang and Jin Q. Cheng -- Apoptotic caspase activation and activity / Jean-Bernard Denault and Guy S. Salvesen -- Biochemical analysis of the native trail death-inducing signaling complex / Henning Walczak and Tobias L. Haas -- Laser microdissection sample preparation for RNA analyses / Christopher J. Vega
Summary Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years. However, as our knowledge of the intricate intracellular events increase, we realize the complexity of the regulatory mechanisms that control it. As such, Apoptosis and Cancer describes the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. This book is a collaboration between academics- and industry-based scientists. The chapters have the technical development characteristic of an academic environment and the standardized system necessary for translating a product to general use, as provided by the industry. Thus, the protocols described within this groundbreaking new volume will aid both an academic laboratory interested in further characterizing the mechanism of apoptosis and an industry laboratory interested on identifying new target molecules or screening for new compounds with potential clinical use. The authors - all leaders in the field - have created a comprehensive text that covers the newest available techniques as well as conventional basic techniques. Apoptosis and Cancer will undoubtedly provide the necessary resources to everyone interested in the area of apoptosis
Analysis apoptose
apoptosis
neoplasms
protocollen
protocols
Cellular Biology
Celbiologie
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Carcinogenesis.
Apoptosis.
Cancer cells.
Tumors -- Growth -- Regulation
Apoptosis -- physiology
Caspases
Neoplasms -- etiology
Neoplasms -- physiopathology
Apoptosis
Carcinogenesis
Apoptosis
Cancer cells
Carcinogenesis
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Mor, Gil.
Alvero, Ayesha B.
ISBN 9781597453394
1597453390