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Title Auditory and vestibular research : methods and protocols / edited by Bernd Sokolowski
Published New York, N.Y. : Humana ; [London] : [Springer, distributor], ©2009


Description 1 online resource (xii, 421 pages) : illustrations (some color)
Series Springer protocols
Methods in molecular biology, 1064-3745 ; 493
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 493
Contents RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio [and others] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande [and others] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico [and others] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan [and others] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith [and others] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada
Summary While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Auditory and Vestibular Research: Methods and Protocols is an ideal guide to the field and a useful tool for exploring genes and proteins in other systems as well, especially systems in which tissues are scarce and a comparative approach lends itself to discovering the underlying causes of human disorders
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Vestibular apparatus.
Hearing disorders.
Equilibrium (Physiology)
Auditory Pathways -- physiology
Ear Diseases -- diagnosis
Vestibular Diseases -- diagnosis
Vestibule, Labyrinth -- physiology
Hearing Disorders
Equilibrium (Physiology)
Hearing disorders
Vestibular apparatus
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Sokolowski, Bernd
ISBN 9781597455237