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Book Cover
Author Hoffman, Robert M

Title Patient-Derived Mouse Models of Cancer : Patient-Derived Orthotopic Xenografts (PDOX)
Published Cham : Springer International Publishing, 2017


Description 1 online resource (304 pages)
Series Molecular and Translational Medicine
Molecular and translational medicine.
Contents Preface; Contents; List of Contributors; 1: In Memoriam: Jørgen Rygaard Ư (1934-2016), Father of Patient-Derived Mouse Models of Cancer and Modern Cancer Research; Patient-Derived Orthotopic Xenografts (PDOX); Transgenic Nude Mice Expressing Fluorescent Proteins in Almost All Tissues; References; 2: The Revival of Patient-Derived Xenograft Mouse Models of Cancer: Way Back to the Future; References; 3: Patient-Derived Xenograft Models for Human Cancer: The Freiburg Experience; Historical Aspects of PDX; Origin and Properties of Nude Mouse; Morphologic and Physiologic Characteristics
Immunological Properties Early Freiburg Experience in the Establishment, Characterization, and Therapeutic Use of PDX from 1978 Until 1988; Take Rate, Growth Behavior, and Drug Response from 1979 Until 1988; PDX Take/Growth as a Prognostic Factor for Survival of Cancer Patients; PDX Regrowth After Being Frozen in Liquid Nitrogen; Similarities and Changes of PDX Compared to the Donor Tumor; Tumor Models Available in 1991; Comparison of Drug Response of the Tumor Growing in the Nude Mouse and in the Patient; Experimental Design of Testing; Chemotherapy
Evaluation Parameters for Tumor Response Comparison of Tumor Response; Potential of the Nude Mouse System as a Predictive Assay; Tumor Clonogenic Assay as a 3D Culture System to Preselect Sensitive Models; PDX as a Valuable Model System for Tumor Biology Studies; Combined In Vitro and In Vivo Testing Procedure; In Vitro and In Vivo Evaluation of Anticancer Agents from a German Drug Development Program, the EORTC and the NCI; The Concept of Preclinical In Vivo Phase II Studies; Molecular Characterization of the PDX Panel
Bevacizumab as an Example of the Development of a Predictive Gene Signature PDX for the Identification of Targeted Therapy, Example: B-raf- Inhibitor Vemurafenib; Outlook; PDX for Anticancer Drug Development; PDX for Patient-Oriented Sensitivity Testing; Summary; References; 4: From Ectopic to Orthotopic Tumor Grafting Sites: Evidence for a Critical Role of the Host Tissue Microenvironment for the Actual Expression of the Malignant Phenotype; An Historical Account; The Popular Subcutaneous Route; The Orthos Way; Comments in Conclusion; References
5: The Effects of the Organ Microenvironment on Metastatic Cell Gene Signatures Introduction; Process of Metastasis; Cross-Species Hybridization of Microarrays for Studies of Metastasis; Experimental Approach; Cancer Type-Specific Gene Signatures; Organ Type-Specific Gene Signatures: Effects of the Organ Microenvironment on Cancer Cell Gene Signatures; Organ and Cancer Type-Specific Gene Signatures; Additional Studies; References; 6: Techniques for Surgical Orthotopic Implantation of Human Tumors to Immunodeficient Mice; Introduction; Early Nude-Mouse Models of Human Cancer
Summary This text highlights seminal discoveries and also provides comprehensive and state-of the-art approach to mouse models of human patient tumors. These areas include training, basic techniques, as well as general troubleshooting. Subsequent chapters focus on the different mouse models of patient tumors including the various strains of immunodeficient mice currently available and the transplantation techniques that can be used as well as state-of-the-art imaging techniques. Practical applications of the models from drug discovery, genome analysis to personalized treatment are also covered. Written by experts in that field, each of these sections address these critical issues. A brief review of the existing literature addressing the particular topic follows in each section. Presently, there is no single source to provide information on technique and uses of mouse models of human patient tumors. Patient-Derived Mouse Models of Cancer will satisfy this need for cancer researchers, oncologists, pharmaceutical and biotechnology industry scientists as well as molecular biologists studying in vivo systems
Notes Surgical Orthotopic Implantation (SOI) of Tumor Fragments
Print version record
Subject Cancer -- Animal models.
Diseases -- Animal models.
Xenograft Model Antitumor Assays
Disease Models, Animal
Transplantation, Heterologous -- methods
Transplantation, Heterologous
MEDICAL -- Forensic Medicine.
MEDICAL -- Preventive Medicine.
MEDICAL -- Public Health.
Cancer -- Animal models.
Genre/Form Electronic book
Form Electronic book
ISBN 3319574248