| Description |
1 online resource (xi, 280 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 234 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 234
|
| Contents |
Adenovirus expressing p53 / M. Holmes, E. Rosenberg and K. Valerie -- Purification of recombinant p53 from sf9 insect cells / X.Z. Sun, J. Nguyen and J. Momand -- Generation and characterization of p53 mutant mice / M.L. Dumble, L.A. Donehower and X. Lu -- Identification of p53 target genes by fluorescent differential display / S. Stein [and others] -- P53-induced gene expression analysis / J.K. Sax and W.S. El-Deiry -- Real-time polymerase chain reaction quantitation of relative expression of genes modulated by p53 using sybr green i / K.E. Stagliano, E. Carchman and S. Deb -- Transactivation and transrepression studies with p53 / M.J. Scian [and others] -- Methods to study p53-repressed promoters / P. Dumont, A. Della Pietra and M.E. Murphy -- Interaction of p53 with cellular proteins / D.M. Keller, S.X. Zeng and H. Lu -- Interaction of p53 with the adenovirus e1b-55 kda protein / J. Roth and M. Dobbelstein -- In vivo footprinting and DNA affinity chromatography for analysis of p53 DNA binding ability / M.P. Molina, C. Cain and J. Bargonetti -- Signaling to p53: The use of phospho-specific antibodies to probe for in vivo kinase activation / A.L. Craig [and others] -- Detection of mitochondrial localization of p53 / M. Mihara and U.M. Moll -- Analysis of nuclear and cytoplasmic degradation of p53 in cells after stress / T.W. Joseph and U.M. Moll -- Mutational analysis of p53 in human tumors: Direct DNA sequencing and sscp / S. Erster, N. Slade and U.M. Moll -- Mutational analysis of p53 in human tumors: Immunocytochemistry / N. Slade and U.M. Moll -- Flow cytometric analysis of p53-induced apoptosis / M. Berger and Y. Haupt -- Flow cytometric analysis of mdm2-mediated growth arrest / R. Frum and S.P. Deb |
| Summary |
Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. Described by leading basic and clinical researchers who have successfully used the methods, the techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. Special effort has been made to discuss problems and their solutions to help ease the learning curve when standardizing a new method. Diverse and highly practical, p53 Protocols offers both beginning and experienced researchers in cancer biology a gold-standard compendium of readily reproducible techniques for studying p53 and related proteins |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
p53 protein -- Laboratory manuals
|
|
p53 antioncogene -- Laboratory manuals
|
|
Protein p53 -- analysis
|
|
Protein p53 -- therapeutic use
|
|
Tumor Suppressor Proteins -- analysis
|
|
Genes, p53 -- physiology
|
|
Neoplasms -- genetics
|
|
MEDICAL -- Biochemistry.
|
|
p53 antioncogene
|
|
p53 protein
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Deb, Sumitra.
|
|
Deb, Swati Palit.
|
| ISBN |
9781592594085 |
|
1592594085 |
|
1588291065 |
|
9781588291066 |
|
1280842814 |
|
9781280842818 |
|
9786610842810 |
|
6610842817 |
|
