| Description |
1 online resource (x, 238 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; v. 962 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 962. 1064-3745
|
| Contents |
Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues / Marie P. Khoury [and others] -- Detecting p53 isoforms at protein level / Virginie Marcel [and others] -- Autophagy, senescence, and apoptosis / Rachel W. Goehe [and others] -- p53 and cell cycle effects after DNA damage / Emir Senturk and James J. Manfredi -- p53 ubiquitination and proteasomal degradation / Ian M. Love, Dingding Shi, and Steven R. Grossman -- Identification of p53 in mitochondria / Angelina V. Vaseva and Ute M. Moll -- Identification of novel mutant p53 interacting proteins by proteomic analysis / Sumitra Deb and Paul R. Graves -- Identification of small molecules affecting p53-MDM2/MDMX interaction by fluorescence polarization / Qi Zhang and Hua Lu -- Determine the effect of p53 on chemosensitivity / Emir Senturk and James J. Manfredi -- Measurement of chemosensitivity and growth rate in p53 expressing cells / Mahesh Ramamoorthy [and others] -- Mutant p53 in cell adhesion and motility / W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb -- Use of the DNA fiber spreading technique to detect the effects of mutant p53 on DNA replication / Rebecca A. Frum, Sumitra Deb, and Swati Palit Deb -- Generation of p53-deficient induced pluripotent stem cells from mouse embryo fibroblasts / Evguenia M. Alexandrova and Ute M. Moll -- p53 actions on microRNA expression and maturation pathway / Hiroshi I. Suzuki and Kohei Miyazono -- Isolation and characterization of murine multipotent lung stem cells / Venkat S. Gadepalli, Catherine Vaughan, and Raj R. Rao -- Generation of p53 knock-down cell lines / Catherine Vaughan, Swati Palit Deb, and Sumitra Deb -- ChIP for identification of p53 responsive DNA promoters / Jun-Ming Liao and Hua Lu -- ChIP-on-chip to identify mutant p53 targets / Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino -- ChIP sequencing to identify p53 targets / Catherine Vaughan, Brad Windle, and Sumitra Deb |
| Summary |
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls |
|
Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors |
| Analysis |
stamcellen |
|
stem cells |
|
veroudering |
|
senescence |
|
dna replication |
|
responsgenen |
|
target genes |
|
protocollen |
|
protocols |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Subject |
p53 protein -- Laboratory manuals
|
|
p53 antioncogene -- Laboratory manuals
|
|
Genes, p53 -- physiology
|
|
Tumor Suppressor Protein p53 -- analysis
|
|
Neoplasms -- genetics
|
|
Tumor Suppressor Proteins -- analysis
|
|
p53 antioncogene
|
|
p53 protein
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Deb, Sumitra.
|
|
Deb, Swati Palit.
|
| ISBN |
9781627032360 |
|
1627032363 |
|
