| Description |
1 online resource (xlv, 544 pages, 10 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in enzymology, 0076-6879 ; v. 424 |
|
Methods in enzymology ; v. 424. 0076-6879
|
| Contents |
Isolation and compositional analysis of trypanosomatid editosomes / A.K. Panigrahi ... [et al.] -- Uridine insertion/deletion editing activities / J. Carnes ... [et al.] -- RNA editing uridylyltransferases of trypanosomatids / R. Aphasizhev ... [et al.] -- Isolation of RNA binding proteins involved in insertion/deletion editing / M. Pelletier ... [et al.] -- RNA-protein interactions in assembled editing complexes in trypanosomes / J. Cruz-Reyes ... [et al.] -- Strategies of kinetoplastid cryptogene discovery and analysis / D.A. Maslov ... [et al.] -- RNA editing in Physarum mitochondria: Assays and biochemical approaches / E.M. Byrne ... [et al.] -- Computational approaches to insertional RNA editing / R. Bundschuh ... [et al.] -- Evolution of RNA editing sites in the mitochondrial small subunit rRNA of the Myxomycota / U. Krishnan ... [et al.] -- Analysis of 5'- or 3'-terminal trna editing: Mitochondrial 5' tRNA editing in Acanthamoeba castellanii as the exemplar / A.J. Lohan ... [et al.] -- Comparative genomic and bioinformatic approaches for the identification of new adenosine-to-inosine substrates / J. Sixsmith ... [et al.] -- Genetic approaches to studying adenosine-to-inosine RNA editing / J.E.C. Jepson ... [et al.] -- A method for finding sites of selective adenosine deamination / J. Ohlson ... [et al.] -- Purification and assay of ADAR activity / L.P. Keegan ... [et al.] -- Large-scale overexpression and purification of ADARs from Saccharomyces cerevisiae for biophysical and biochemical studies / M.R. Macbeth ... [et al.] -- Mouse models to elucidate the functional roles of adenosine-to-inosine editing / E.Y. Rula ... [et al.] -- Probing adenosine-to-inosine editing reactions using RNA-containing nucleoside analogs / O. Maydanovych ... [et al.] -- Measuring editing activity and identifying cytidine-to-uridine mrna editing factors in cells and biochemical isolates / H.C. Smith ... [et al.] -- Mouse models as tools to explore cytidine-to-uridine RNA editing / S. Cho ... [et al.] -- RNA editing in plant mitochondria: Assays and biochemical approaches / M. Takenaka ... [et al.] -- Assay of editing of exogenous RNAs in chloroplast extracts of Arabidopsis, maize, pea, and tobacco / M.L. Hayes ... [et al.] -- In organello gene expression and RNA editing studies by electroporation-mediated transformation of isolated plant mitochondria / J. Farre ... [et al.] -- Transformation of the plastid genome to study RNA editing / K.A. Lutz ... [et al.] |
| Summary |
RNA processing plays a critical role in realizing the full potential of a given genome. One means of achieving protein diversity is through RNA editing. A diverse array of editing events has been characterized, affecting gene expression in organisms from viruses and single cell parasites to humans and plants. The variety of editing mechanisms has required the development of many different experimental approaches, many of which are likely to be broadly applicable, particularly given the interplay between editing and other cellular processes, including transcription, splicing, and RNA silencing. This volume not only covers most of the principal methods employed in the field, but also offers innovative solutions to the significant challenges posed by these experimental systems. * Presents newly developed methods * Covers topics ranging from biochemistry to bioinformatics * Includes innovative solutions to potential problems |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
RNA editing.
|
| Form |
Electronic book
|
| Author |
Gott, Jonatha M.
|
| ISBN |
008055105X (electronic bk.) |
|
0123739225 (electronic bk.) |
|
9780080551050 (electronic bk.) |
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9780123739223 (electronic bk.) |
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