A primer on transcriptional regulation in mammalian cells -- Initial strategic issues -- Modes of regulating mRNA abundance -- Transcription initiation site mapping -- Functional assays for promoter analysis -- Identification and analysis of distant control regions -- Identifying cis-acting DNA elements within a control region -- Identification of DNA-binding proteins and isolation of their genes -- Confirming the functional importance of a protein-DNA interaction -- In vivo analysis of an endogenous control region -- Approaches for the synthesis of recombinant transcription factors -- Identifying and characterizing transcription factor domains -- Theory, characterization, and modeling of DNA binding by regulatory transcription factors -- Crude and fractionated systems for in vitro transcription -- Approaches for studying transcription complex assembly