| Description |
1 online resource (54 p.) |
| Contents |
Multiplexing of PCR Assays in Breast Cancer Analysis; Table of Contents; 1 Introduction; 1.1 Breast Cancer; 1.2 RNA Expression Profiling and Prediction; 1.3 Objectives; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments and Consumables; 2.1.2 Buffers and Chemicals for Nucleic Acid Purification; 2.1.3 Nucleic Acids, Cell Lines & Tissue Samples; 2.1.4 Synthetic Oligonucleotides; 2.1.5 Chemicals and Kits for PCR; 2.1.6 Software; 2.2 Methods; 2.2.1 General Approach; 2.2.2 Purification of Nucleic Acids; 2.2.3 Creation of a Standard Reference RNA Pool; 2.2.4 Real-Time Kinetic RT-PCR |
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2.2.5 Designing Dually Labeled Primer-Probe Sets2.2.6 Dilution of Primer-Probe Sets; 2.2.7 Setup of Singleplex kPCR Assays; 2.2.8 Evaluation of Different Reporter Dyes; 2.2.9 Controlling of Primer-Probe-Set Performance; 2.2.10 Testing Combinations of Two Primer-Probe Sets (Duplexing); 2.2.11 Testing Combinations of Three Sets (Triplexing); 3 Results; 3.1 Identification of Suitable Reporter Dyes; 3.2 Evaluation of Primer-Probe-Performance; 3.3 Identification of Suitable Duplex Combinations; 3.4 Identification of Suitable Triplex Combinations; 4 Discussion |
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4.1 Statistical Evaluation of Primer Performances4.1.1 Slope; 4.1.2 Efficiency; 4.1.3 Y-Intercept; 4.2 Suitable Reporter Dyes and Quencher; 4.3 Optimization of Assays and kinetic RT-PCR Parameter; 4.4 Conclusion and Outlook; 5 Summary; 6 Literature; 7 Appendix; 7.1 Abbreviations; 7.2 Figures; 7.3 Tables; 7.4 Oligonucleotide Sequences; 7.5 Origins of Breast Cancer Samples for MAVPOOL080623a; 7.6 Acknowledgements |
| Summary |
HauptbeschreibungDas Werk beschreibt die Optimierung eines prognostischen molekularbiologischen Tests für die Brustkrebs-Diagnose und -Therapieentscheidung. Im Rahmen der Arbeit wurde die Zahl der Reaktionsräume für Polymerasekettenreaktionen (PCR) durch die Optimierung vom Multiplexen derart reduziert, dass parallele und kosteneffizientere Analysen möglich wurden. Biographische InformationenPatrick Maaß, B.Sc., wurde 1973 in Wattenscheid geboren. Nach einer Ausbildung zum Biologielaboranten bei einem großen deutschen Pharmaforschungsunternehmen entschied sich der |
| Notes |
Description based upon print version of record |
| Bibliography |
Includes bibliographical references |
| Notes |
English |
| Subject |
Breast -- Cancer -- Diagnosis
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Breast -- Cancer -- Imaging
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Breast -- Cancer -- Diagnosis
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Breast -- Cancer -- Imaging
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| Form |
Electronic book
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| ISBN |
3863416074 |
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9783863416072 |
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