| Description |
1 online resource (xiii, 314 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1461 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; . 1461
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Springer protocols (Series)
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| Contents |
Label-free cell phenotypic identification of D-luciferin as an agonist for GPR35 / Heidi Hu, Huayun Deng, and Ye Fang -- Synthetic bioluminescent coelenterazine derivatives / Ryo Nishihara, Daniel Citterio, and Koji Suzuki -- Molecular cloning of secreted luciferases from marine planktonic copepods / Yasuhiro Takenaka, Kazuho Ikeo, and Yasushi Shigeri -- How to fabricate functional artificial luciferases for bioassays / Sung-Bae Kim and Rika Fujii -- Quantum yield determination based on photon number measurement, protocols for firefly bioluminescence reactions / Kazuki Niwa -- Bioluminescent ligand-receptor binding assays for protein or peptide hormones / Ya-Li Liu and Zhan-Yun Guo -- Bioluminogenic imaging of aminopeptidase N in vitro and in vivo / Wenxiao Wu [and others] -- Firefly luciferase-based sequential bioluminescence resonance energy transfer (BRET)-fluorescence resonance energy transfer (FRET) protease assays / Bruce Branchini -- Monitoring intracellular pH change with a genetically encoded and ratiometric luminescence sensor in yeast and mammalian cells / Yunfei Zhang [and others] -- Protein-protein interaction assay FlimPIA based on the functional complementation of mutant firefly luciferases / Yuki Ohmuro-Matsuyama and Hiroshi Ueda -- Single-chain probes for illuminating androgenicity of chemicals / Sung- Bae Kim and Hiroaki Tao -- Multicolor imaging of bifacial activities of estrogens / Sung-Bae Kim and Yoshio Umezawa -- Circular permutation probes for illuminating phosphorylation of estrogen receptor / Sung- Bae Kim and Hiroaki Tao -- Fabrication of molecular strain probes for illuminating protein-protein interactions / Sung-Bae Kim and Rika Fujii -- ALuc-based molecular tension probe for sensing intramolecular protein-protein interactions / Sung-Bae Kim, Ryo Nishihara, and Koji Suzuki -- Live cell bioluminescence imaging in temporal reaction of G protein-coupled receptor for high-throughput screening and analysis / Mitsuru Hattori and Takeaki Ozawa -- Imaging histone methylations in living animals / Thillai V. Sekar and Ramasamy Paulmurugan -- Preparation and assay of simple light off biosensor based on immobilized bioluminescent bacteria for general toxicity assays / G.V.M. Gabriel and V.R. Viviani -- In vivo bioluminescent imaging of ATP-binding cassette transporter-mediated efflux at the blood-brain barrier / Joshua Bakhsheshian [and others] -- Theranostic imaging of cancer gene therapy / Thillai V. Sekar and Ramasamy Paulmurugan -- Development of a multicolor bioluminescence imaging platform to simultaneously investigate transcription factor NF-?B signaling and apoptosis / Vicky T. Knol-Blankevoort [and others] -- Multichannel bioluminescence determination platform for bioassays / Sung-Bae Kim and Ryuichi Naganawa -- Bioluminescence assay system for imaging metal cationic activities in urban aerosols / Sung-Bae Kim [and others] -- Luminescence imaging : (a) multicolor visualization of Ca2+ dynamics in different cellular compartments and (b) video-rate tumor detection in a freely moving mouse / Kenta Saito, Masahiro Nakano, and Takeharu Nagai -- Photon counting system for high-sensitivity detection of bioluminescence at optical fiber end / Masataka Iinuma, Yutaka Kadoya, and Akio Kuroda |
| Summary |
Annotation This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. It guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Bioluminescence.
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Bioluminescence assay.
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Medical laboratory technology.
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Luminescent Measurements
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Biosensing Techniques
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Clinical Laboratory Techniques
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Biochemistry.
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Science -- Life Sciences -- Biochemistry.
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Medical laboratory technology
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Bioluminescence
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Bioluminescence assay
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| Form |
Electronic book
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| Author |
Kim, Sung-Bae
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| ISBN |
9781493938131 |
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1493938134 |
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1493938118 |
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9781493938117 |
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