| Description |
xv, 611 pages : illustrations ; 24 cm |
| Contents |
1. [delta]-(L-[alpha]-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase as a Model Tripeptide Synthetase / Hans von Dohren, Wibke Kallow and Mary Anne Tavanlar / [et al.] -- 2. Metabolic Engineering for Cephalosporin C. Yield Improvement and Production of Intermediates / Joe E. Dotzlaf, Steven W. Queener and Wu-Kuang Yeh -- 3. Bioconversion of Penicillins to Cephalosporins / Arnold L. Demain, Jose L. Adrio and Jacqueline M. Piret -- 4. Direct Fermentative Production of Acyltylosins by Genetically Engineered Strains of Streptomyces fradiae / Akira Arisawa and Hiroshi Tsunekawa -- 5. Engineering Streptomyces avermitilis for the Production of Novel Avermectins: Mutant Design and Titer Improvement / Claudio D. Denoya, Kim J. Stutzman-Engwall and Hamish A. I. McArthur -- 6. Biocatalytic Syntheses of Chiral Intermediates for Antihypertensive Drugs / Ramesh N. Patel -- 7. Cloning, Structure, and Activity of Ketone Reductases from Baker's Yeast / Jon D. Stewart, Sonia Rodriguez and Margaret M. Kayser -- 8. Cross-Linked Enzyme Crystals: Biocatalysts for the Organic Chemist / Michael D. Grim -- 9. Enzymatic Deacylation of Echinocandins and Related Antifungal Agents / Andrew R. Cockshott, Adam J. Kreuzman and Wu-Kuang Yeh -- 10. Roles of Enzymes in Antibacterial Drug Discovery / Siddhartha Roychoudhury -- 11. Penicillin-Binding Proteins as Antimicrobial Targets: Expressing, Purification, and Assay Technologies / Genshi Zhao, Timothy I. Meier and Wu-Kuang Yeh -- 12. Development of a High-Throughput Screen for Streptococcus pneumoniae UDP-N-Acetylmuramoyl-Alanine: D-Glutamate Ligase (MurD) for the Identification of MurD Inhibitors / Michele C. Smith, Jams A. Cook and Gary M. Birch / [et al.] -- 13. Purification and Assay Development for Human Rhinovirus Proteases / Q. May Wang and Robert B. Johnson -- 14. Screening for Parasiticides Using Recombinant Microorganisms / Timothy G. Geary -- 15. Screening for Inhibitors of Lipid Metabolism / Hiroshi Tomoda and Satoshi Omura -- 16. Design and Development of a Selective Assay System for the Phospholipase A[subscript 2] Superfamily / Hsiu-Chiung Yang, Marian Mosior and Edward A. Dennis -- 17. Understanding an Exploiting Bacterial Polyketide Synthases / Robert McDaniel and Chaitan Khosla -- 18. Polyketide Synthases: Analysis and Use in Synthesis / Kira J. Weissman and James Staunton -- 19. Enzymatic Synthesis of Fungal N-Methylate Cyclopeptides and Depsipeptides / Mirko Glinski, Till Hornbogen and Rainer Zocher -- 20. New Strategies for Target Identification, Validation, and Use of Enzymes in High-Throughput Screening / Joaquim Trias and Zhengyu Yuan -- 21. Use of Genomics for Enzyme-Based Drug Discovery / Molly B. Schmid -- 22. Assigning Precise Function to Genes / Ridong Chen -- 23. Redesigning Binding and Catalytic Specificities of Enzymes / Ridong Chen -- 24. Proteomics: Chromatographic Fractionation Prior to Two-Dimensional Polyacrylamide Gel Electrophoresis for Enrichment of Low-Abundance Proteins to Facilitate Identification by Mass Spectrometric Methods / Srinivasan Krishnan, John E. Hale and Gerald W. Becker |
| Summary |
"This reference provides a review of enzyme functions in human and animal health - covering basic principles and applications in antibiotic biosynthesis, biocatalysis, and screening and assay optimization, as well as new and emerging technologies in the biotechnological and pharmaceutical industries." "Emphasizing modern methods of metabolic engineering and drug discovery processes, Enzyme Technologies for Pharmaceutical and Biotechnological Applications details enzyme targets related to human disease ... the development of effective enzymatic assays ... up-to-the-minute methodologies in high-throughput screening ... the discovery of novel compounds for lead generation ... improvements in antibiotic yield ... the generation of contemporary protein products ... and more."--BOOK JACKET |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Enzymes -- Biotechnology.
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Enzymes -- Industrial applications.
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Pharmaceutical biotechnology.
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| Author |
Kirst, Herbert A.
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Yeh, Wu-Kuang, 1942-
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Zmijewski, Milton J., 1948-
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| LC no. |
2001028936 |
| ISBN |
0824705491 alkaline paper |
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