| Description |
1 online resource (xxxi, 681 pages) : illustrations |
| Series |
Methods in enzymology ; v. 185 |
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Methods in enzymology ; v. 185
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| Contents |
Expression of heterologous genes in mammalian cells / Arthur D. Levinson and David V. Goeddel -- Vectors used for expression in mammalian cells / Randal J. Kaufman and David V. Goeddel -- Assembly of enhancers, promoters, and splice signals to control expression of transferred genes / Michael Kriegler and David V. Goeddel -- Methods for introducing DNA into mammalian cells / Wayne A. Keown ... [et al.] -- Selection and coamplification of heterologous genes in mammalian cells / Randal J. Kaufman and David V. Goeddel -- Optimizing cell and culture environment for production of recombinant proteins / Jennie P. Mather and David V. Goeddel -- Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells / Andrew J. Dorner, Kaufman Randal J. and David V. Goeddel -- Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil / Bruce H. Horwitz, Daniel Dimaio and David V. Goeddel |
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Heterologous gene expression in yeast / Scott D. Emr and David V. Goeddel -- Propagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors / Alan B. Rose, James R. Broach and David V. Goeddel -- Manipulating yeast genome using plasmid vectors / Tim Stearns ... [et al.] -- Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast / Lawrence M. Mylin ... [et al.] -- Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter / Virginia L. Price ... [et al.] -- Induced expression using yeast copper metallothionein promoter / Tina Etcheverry and David V. Goeddel -- High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene / S. M. Kingsman ... [et al.] -- Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins / Steven Rosenberg ... [et al.] -- Superimposition of temperature regulation on yeast promoters / Andrzej Z. Sledziewski ... [et al.] -- Sequence and structural requirements for efficient translation in yeast / Thomas F. Donahue, Mark A. Cigan and David V. Goeddel -- Vacuolar proteases in yeast Saccharomyces cerevisiae / Elizabeth W. Jones and David V. Goeddel -- Detection and inhibition of ubiquitin-dependent proteolysis / Keith D. Wilkinson and David V. Goeddel -- Contranslational amino-terminal processing / Richard L. Kendall ... [et al.] -- [alpha]-Factor leader-directed secretion of heterologous proteins from yeast / Anthony J. Brake and David V. Goeddel -- Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast / Ronald A. Hitzeman ... [et al.] -- Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects / Clinton E. Ballou and David V. Goeddel -- Molecular and genetic approach to enhancing protein secretion / Vanessa Chisholm ... [et al.] -- |
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Systems for heterologous gene expression / David V. Goeddel and David V. Goeddel -- Expression of heterologous proteins in escherichia coli / Larry Gold and David V. Goeddel -- Design and construction of expression plasmid vectors in escherichia coli / Paulina Balbas, Francisco Bolivar and David V. Goeddel -- Promoter recognition and mRNA initiation by Escherichia coli E[sigma]70 / Jay D. Gralla and David V. Goeddel -- Use of Escherichia coli trp promoter for direct expression of proteins / Daniel G. Yansura, Dennis J. Henner and David V. Goeddel -- Use of T7 RNA polymerase to direct expression of cloned genes / F. William Studier ... [et al.] -- High-level translation initiation / Larry Gold, Gary D. Stormo and David V. Goeddel -- Enhanced translational efficiency with two-cistron expression system / Brigitte E. Schoner ... [et al.] -- Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species / Herman A. De Boer, Anna S. Hui and David V. Goeddel -- Vector for enhanced translation of foreign genes in Escherichia coli / Peter O. Olins, Shaukat H. Rangwala and David V. Goeddel -- Minimizing proteolysis in Escherichia coli: genetic solutions / Susan Gottesman and David V. Goeddel -- Gene fusions for purpose of expression: An introduction / Mathias Uhlén, Tomas Moks and David V. Goeddel -- Fusions to staphylococcal protein A / Björn Nilsson, Lars Abrahmsén and David V. Goeddel -- Expression as trpE fusion / Daniel G. Yansura and David V. Goeddel -- Engineering Escherchia coli to secrete heterologous gene products / Joan A. Stader, Thomas J. Silhavy and David V. Goeddel -- Refolding of recombinant proteins / Tadahiko Kohno ... [et al.] -- Expression of heterologous genes in Bacillus subtilis / Dennis J. Henner and David V. Goeddel -- Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis / Stuart F. J. Le Grice and David V. Goeddel -- System for secretion of heterologous proteins in Bacillus subtilis / Vasantha Nagarajan and David V. Goeddel -- Inducible expression of regulatory genes in Bacillus subtilis / Dennis J. Henner and David V. Goeddel -- |
| Summary |
The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter |
| Analysis |
Genetic engineering |
| Notes |
Includes indexes |
| Bibliography |
Includes bibliographical references |
| Notes |
Description based on print version record |
| Subject |
Gene expression -- Technique.
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Molecular cloning.
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Cloning, Molecular.
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Gene Expression.
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Genetic Techniques.
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| Form |
Electronic book
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| Author |
Goeddel, David V.
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| ISBN |
0121820866 (electronic bk.) |
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9780121820862 (electronic bk.) |
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