| Description |
1 online resource (xxxviii, 540 pages, 32 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in enzymology, 0076-6879 ; v. 375 |
|
Methods in enzymology ; v. 375. 0076-6879
|
| Contents |
1. Mining core histone sequences from public protein databases -- 2. Reconstitution of nucleosome core particles from recombinant histones and DNA -- 3. Preparation and crystallization of nucleosome core particle -- 4. Creating designer histones by native chemical ligation -- 5. Two-dimensional gel analysis of histones and other H2AX-related methods -- 6. Assembly of yeast chromatin using ISWI complexes -- 7. Reconstitution of yeast chromatin using yNap1p -- 8. DNA synthesis-dependent and -independent chromatin assembly pathways in Xenopus egg extracts -- 9. Preparation of defined mononucleosomes, dinucleosomes, and nucleosome arrays in vitro and analysis of transcription factor binding -- 10. Purification of native, defined chromatin segments -- 11. Purification of defined chromosomal domains -- 12. Probing core histone tail-DNA interactions in a model dinucleosome system -- 13. Site-directed histone-DNA contact mapping for analysis of nucleosome dynamics -- 14. Site-specific attachment of reporter compounds to recombinant histones -- 15. Histone MacroH2A purification and nucleosome reconstitution -- 16. Analysis of histone variant H2A.Z localization and expression during early development -- 17. Histone variant CENP-A purification, nucleosome reconstitution -- 18. Immunological analysis and purification of centromere complex -- 19. Purification and analyses of histone H1 variants and H1 posttranslational modifications -- 20. HMGA proteins: isolation, biochemical modifications, and nucleosome interactions -- 21. Preparation and functional analysis of HMGN proteins -- 22. Methods for visualizing chromatin dynamics in living yeast -- 23. Direct visualization of transcription factor-induced chromatin remodeling and cofactor recruitment in vivo -- 24. Measuring histone and polymerase dynamics in living cells -- 25. Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy -- 26. Quantification of protein-protein and protein-DNA interactions in vivo, using fluorescence recovery after photobleaching -- 27. Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei -- 28. Correlative light and electron spectroscopic imaging of chromatin in situ -- 29. RNA fluorscence in situ hybridization tagging and recovery of associated proteins to analyze in vivo chromatin interactions -- 30. 3C technology: analyzing the spatial organization of genomic loci in vivo / Erik Splinter, Frank Grosveld, and Wouter de Laat |
| Summary |
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription. *Histone Bioinformatics *Biochemistry of histones, nucleosomes and chromatin *Molecular cytology of chromatin functions |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Description based on print version record |
| Subject |
Chromatin.
|
|
Chromatin.
|
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Chromatin -- enzymology.
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| Form |
Electronic book
|
| Author |
Allis, C. David.
|
|
Wu, Carl.
|
| ISBN |
0080497136 (electronic bk.) |
|
0121827798 (electronic bk.) |
|
9780080497136 (electronic bk.) |
|
9780121827793 (electronic bk.) |
|
