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Title Signal transduction protocols / edited by David A. Kendall and Steven J. Hill
Published Totowa, N.J. : Humana Press, [1995]
©1995

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Location Call no. Vol. Availability
 W'PONDS  574.876 Ken/Stp  AVAILABLE
Description xi, 305 pages : illustrations ; 23 cm
Series Methods in molecular biology ; v. 41
Methods in molecular biology (Clifton, N.J.) ; v. 41
Contents Machine derived contents note: The Problems and Pitfalls of Radioligand Binding. Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor. Autoradiography of b1- and b2-Adrenoreceptors. In Situ Hybridization. Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes. Measurement of Adenylyl Cyclase Activity in Cell Membranes. The Measurement of Cyclic AMP Levels in Biological Preparations. Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay. Measurement of Cyclic GMP Formation. Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay. Purification and Activation of Cyclic GMP-Dependent Protein Kinase. The Analysis and Assay of Cyclic Neucleotide Phosphodiesterase Isoenzyme Activity. Separation of Labeled Inositiol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC). Mass Measurement of Key Phosphoinositide Cycle Intermediates. Measurement of Phospholipase C Activity in Brain Membranes. Measurement of Diacylglycerol: Origins of Receptor-Stimulated Diacylglycerol Accumulation. Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2. Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3. Single-Cell Calcium Imaging. Characterization of Calcium/Calmodulin-Stimulated Proteinase Kinase II. Protein Kinase C: Measurement of Translocation, Activation, and Role in Cellular Responses. The Measurement of Phospholipase D-Linked Signaling in Cells. Phospholipase A2 Activity. Techniques for the Measurement of Nitric Oxide. Index
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press
Subject Cell receptors -- Research -- Methodology.
Cellular signal transduction -- Research -- Methodology.
G proteins -- Research -- Methodology.
Cell receptors -- Research -- Methodology.
Cellular signal transduction -- Research -- Methodology.
G proteins -- Research -- Methodology.
Author Hill, Steven J.
Kendall, David A.
Humana Press.
LC no. 95002769
ISBN 0896032981