Title Mitochondrial DNA : methods and protocols / edited by William C. Copeland

Published Totowa, N.J. : Humana Press, ©2002

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Description 1 online resource (xiv, 414 pages) : illustrations Series Methods in molecular biology ; v. 197 Methods in molecular biology (Clifton, N.J.) ; v. 197 Contents pt. 1. Methods for the analysis of mtDNA -- Animal models for mitochondrial disease / Douglas C. Wallace -- Identification of mutations in mtDNA from patients suffering mitochondrial diseases / Eric A. Schon -- Screening for aging-dependent point mutations in mtDNA / Yuichi Michikawa -- Scanning low-frequency point mutants in the mitochondrial genome using constant denaturant capillary electrophoresis / Weiming Zheng -- Identification of mtDNA mutations in human cancer / Shuji Nomoto -- In situ PCR of the common human mt DNA deletion: is it related to apoptosis? / Steven J Zullo -- Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler / Alice Wong -- Yeast nuclear genes for mtDNA maintenance / Francoise Foury -- Measuring mtDNA mutation rates in Saccharomyces cerevisiae using the mtArg8 assay / Micheline K. Strand -- Measuring oxidative mtDNA damage and repair using quantitative PCR / Janine H. Santos -- Migration of mtDNA into the nucleus / Mary K. Thorsness -- 2D gel electrophoresis of mtDNA / Heather E. Lorimer -- pt. 2. Methods for the analysis of proteins involved in mtDNA transactions -- Purifications of mitochondria for enzymes involved in nucleic acid transactions / Daniel F. Bogenhagen -- Purification of mitochondrial uracil-DNA glycosylase using Ugi-sepharose affinity chromatography / Samuel E. Bennett -- Characterization of specialized mtDNA glycosylases / Simon G. Nyaga -- Purification, separation, and identification of the human mtDNA polymerase with and without its accessory subunit / Matthew J. Longley -- Assay of mtDNA polymerase y from human tissues / Robert K. Naviaux -- Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form: DNA polymerase y / Carol L. Farr -- Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form: mitochondrial single-stranded DNA-binding protein / Carol L. Farr -- Expression, purification, and in vitro assays of mitochondrial single-stranded DNA-binding protein / Kang Li -- Recombinant yeast mtDNA helicases: purification and functional assays / Silja Kuusk -- Isolation of DNA topoisomerases I and II activities from mitochondria of mammalian heart / Robert L. Low -- Endonuclease G isolation and assays / Robert L. Low -- Targeting DNA repair proteins to mitochondria / Allison W. Dobson -- In organello footprinting of mtDNA / Steven C. Ghivizzani -- Crosslinking of proteins to mtDNA / Brett A. Kaufman -- Breeding and genotyping ot Tfam conditional knockout mice / Mats Ekstrand Summary Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Each readily reproducible protocol includes step-by-step instructions, tips on avoiding pitfalls and extending the method to other situation, and introductory material explaining the theory behind the process. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing the role mtDNA plays in the aging process, apoptosis, and possibly some cancers, and for investigating the cause of mitochondrial dysfunction and disease Bibliography Includes bibliographical references and index Notes English Print version record Subject Mitochondrial DNA -- Laboratory manuals Mitochondrial DNA. DNA, Mitochondrial SCIENCE -- Life Sciences -- Genetics & Genomics. Mitochondrial DNA Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Copeland, William C. (William Chenery) LC no. 2002020564 ISBN 0896039722 9780896039728 0585405387 9780585405384 1592592848 9781592592845 1280830689 9781280830686 9786610830688 6610830681

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