| Description |
1 online resource (xi, 312 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 352 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 352
|
| Contents |
Combinatorial protein design strategies using computational methods / Hidetoshi Kono, Wei Wang, and Jeffery G. Saven -- Global incorporation of unnatural amino acids in Escherichia coli / Jamie M. Bacher and Andrew D. Ellington -- Considerations in the design and optimization of coiled coil structures / Jody M. Mason, Kristian M. Müller, and Katja M. Arndt -- Calcium indicators based on calmodulin-fluorescent protein fusions / Kevin Truong [and others] -- Design and synthesis of artificial zinc finger proteins / Wataru Nomura and Yukio Sugiura -- Monobodies: antibody mimics based on the scaffold of the fibronectin type III domain / Akiko Koide and Shohei Koide -- Engineering site-specific endonucleases / Peter Friedhoff and Alfred Pingoud -- Protein library design and screening: working out the probabilities / Michel Denault and Joelle N. Pelletier -- Protein design by binary patterning of polar and nonpolar amino acids / Luke H. Bradley [and others] -- Versatile DNA fragmentation and directed evolution with nucleotide exchange and excision technology / Sabine C. Stebel, Katja M. Arndt, and Kristian M. Müller -- Degenerate oligonucleotide gene shuffling / Peter L. Bergquist and Moreland D. Gibbs -- M13 bacteriophage coat proteins engineered for improved phage display / Sachdev S. Sidhu, Birte K. Feld, and Gregory A. Weiss -- Ribosome-inactivation display system / Satoshi Fujita, Jing-Min Zhou, and Kazunari Taira -- Compartmentalized self-replication: a novel method for the directed evolution of polymerases and other enzymes / Farid J. Ghadessy and Philipp Holliger -- Synthesis of degenerated libraries of the ras-binding domain of raf and rapid selection of fast-folding and stable clones with the dihydrofolate reductase protein fragment complementation assay / François-Xavier Campbell-Valois and Stephen W. Michnick -- A general method of terminal truncation, evolution, and re-elongation to generate enzymes of enhanced stability / Jochen Hecky [and others] |
| Summary |
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering. The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented. Following the successful Methods in Molecular Biology series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Protein engineering.
|
|
Protein Engineering -- methods
|
|
Protein Engineering
|
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SCIENCE -- Biotechnology.
|
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Protein engineering.
|
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Protein Engineering -- methods.
|
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Protein engineering
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Arndt, Katja M.
|
|
Müller, Kristian M.
|
| ISBN |
1588290727 |
|
9781588290724 |
|
9781597451871 |
|
1597451878 |
|
1280832576 |
|
9781280832574 |
|
9786610832576 |
|
6610832579 |
|
