Title Integrin and cell adhesion molecules : methods and protocols / edited Motomu Shimaoka

Published New York : Humana Press, ©2011

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Description 1 online resource (xvi, 529 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; v. 757 Methods in molecular biology (Clifton, N.J.) ; v. 757. 1064-3745 Contents Overview : assays for studying integrin-dependent cell adhesion / Alexandre Chigaev and Larry A. Sklar -- Cell adhesion assays / Gabriele Weitz-Schmidt and Stephanie Chreng -- Real-time analysis of integrin-dependent transendothelial migration and integrin-independent interstitial motility of leukocytes / Ziv Shulman and Ronen Alon -- Lentiviral gene transfer method to study integrin function in T lymphocytes / Daliya Banerjee and Motomu Shimaoka -- Surface plasmon resonance biosensing in studies of the binding between b₂ integrin I domains and their ligands / Thomas Vorup-Jensen -- Cell-free ligand-binding assays for integrin LFA-1 / Koichi Yuki -- Overview : structural biology of integrins / Guanyuan Fu, Wei Wang, and Bing-Hao Luo -- Protein expression and purification of integrin I domains and IgSF ligands for crystallography / Hongmin Zhang and Jia-huai Wang -- Electron microscopic imaging of integrin / Kenji Iwasaki -- NMR method to study protein-protein interactions / Noritaka Nishida and Ichio Shimada -- Single-molecule methods to study cell adhesion molecules / Joonil Seog -- Overview : imaging in the study of integrins / Christopher V. Carman -- Live imaging of LFA-1-dependent T-cell motility and stop signals / Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher -- Monitoring integrin activation by fluorescence resonance energy transfer / Craig T. Lefort, Young-Min Hyun, and Minsoo Kim -- High-resolution fluorescence microscopy to study transendothelial migration / Christopher V. Carman -- Multiphoton intravital microscopy to study lymphocyte motility in lymph nodes / Thomas T. Murooka and Thorsten R. Mempel -- Overview of integrin signaling in the immune system / Tatsuo Kinashi -- Rap1 and integrin inside-out signaling / Koko Katagiri and Tatsuo Kinashi -- Isolation of focal adhesion proteins for biochemical and proteomic analysis / Jean-Cheng Kuo [and others] -- Talin and signaling through integrins / Mohamed Bouaouina, David S. Harburger, and David A. Calderwood -- Proteomics method for identification of pseudopodium phosphotyrosine proteins / Yingchun Wang and Richard L. Klemke -- Overview : studying integrins in vivo / Clifford A. Lowell and Tanya N. Mayadas -- Method for the generation of conditional gene-targeted mice / Masahiro Yamamoto and Kiyoshi Takeda -- T-cell homing to the gut mucosa : general concepts and methodological considerations / Jaime De Calisto [and others] -- In vivo quantitative proteomics : the SILAC mouse / Sara Zanivan, Marcus Krueger, and Matthias Mann -- Analysis of chemotaxis in Dictyostelium / Huaqing Cai [and others] -- Integrins in tumor angiogenesis and lymphangiogenesis / Philippe Foubert and Judith A. Varner -- PET-radioimmunodetection of integrins : imaging acute colitis using a ⁶⁴64Cu-labeled anti-B-- integrin antibody / Jason L.J. Dearling and Alan B. Packard -- Integrin-targeted nanoparticles for siRNA delivery / Noa Ben-Arie, Ranit Kedmi, and Dan Peer -- Humanized mice for studying human leukocyte integrins in vivo / Sang-Soo Kim [and others] Summary Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative Analysis moleculen molecules celbiologie cellular biology Cellular Biology Celbiologie Notes Includes index Bibliography Includes bibliographical references and index Notes English Subject Cell adhesion molecules -- Laboratory manuals Glycoproteins. Cell surface antigens. Antigens. Membrane proteins. Cell receptors. Proteins. Cell adhesion molecules. Integrins. Biological products. Receptors, Immunologic Membrane Glycoproteins Antigens, Surface Antigens Membrane Proteins Receptors, Cell Surface Glycoproteins Biological Factors Proteins Amino Acids, Peptides, and Proteins Chemicals and Drugs Cell Adhesion Molecules Integrins protein. Proteins Membrane proteins Integrins Glycoproteins Cell surface antigens Cell receptors Antigens Cell adhesion molecules Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Shimaoka, Motomu ISBN 9781617791666 1617791660

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