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Title Genomic imprinting : methods and protocols / edited by Nora Engel
Published New York : Humana Press/Springer, ©2012


Description 1 online resource (xi, 297 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 925
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 925. 1064-3745
Springer protocols. 1949-2448
Contents Uniparental embryos in the study of genomic imprinting / Yong Cheng, Dasari Amarnath, and Keith E. Latham -- Derivation of induced pluripotent stem cells by retroviral gene transduction in mammalian species / Masanori Imamura [and others] -- Generation of trophoblast stem cells / Michael C. Golding -- Immunomagnetic purification of murine primordial germ cells / Emily Y. Smith and James L. Resnick -- Whole genome methylation profiling by immunoprecipitation of methylated DNA / Andrew J. Sharp -- Identification of imprinted loci by transcriptome sequencing / Tomas Babak -- Data mining as a discovery tool for imprinted genes / Chelsea Brideau and Paul Soloway -- Engineering of large deletions and duplications in vivo / Louis Lefebvre -- Methylated DNA immunoprecipitation (MeDIP) from low amounts of cells / Julie Borgel, Sylvain Guibert, and Michael Weber -- Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains / Purnima Singh and Piroska E. Szabo -- Quantitative chromosome conformation capture / Raffaella Nativio, Yoko Ito, and Adele Murrell -- Genome-wide analysis of DNA methylation in low cell numbers by reduced representation bisulfite sequencing / Sebastien A. Smallwood and Gavin Kelsey -- Isolation of RNA and DNA from single preimplantation embryos and a small number of mammalian oocytes for Imprinting Studies / Sarah Rose Huffman, Md Almamun, and Rocio Melissa Rivera -- Generation of cDNA libraries from RNP-derived regulatory noncoding RNAs / Mathieu Rederstorff -- Co-immunoprecipitation of long noncoding RNAs / Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil -- Specialized technologies for epigenetics in plants / Wenyan Xiao -- Computational studies of imprinted genes / Martina Paulsen -- Insights on imprinting from beyond mice and men / Andrew Pask -- Nonmammalian parent-of-origin effects / Elena de la Casa-Esperon
Summary Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting. This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come
Analysis biomedische wetenschappen
humane genetica
human genetics
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Subject Genomic imprinting -- Laboratory manuals
Genomic imprinting.
Genomic Imprinting
Genomic imprinting
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Engel, Nora
LC no. 2012943828
ISBN 9781627030106