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Author Marie-Anaïs, Florence

Title "Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (607 secs.)
Series Immunology and Infection
Summary We describe an experimental setup to visualize with unprecedented high resolution phagosome formation and closure in three dimensions in living macrophages, using total internal reflection fluorescence microscopy. It allows monitoring of the base of the phagocytic cup, the extending pseudopods, as well as the precise site of phagosome scission
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Immunology and Infection
Form Streaming audio