Title Mitochondrial DNA : methods and protocols / edited by Jeffrey A. Stuart

Edition 2nd ed Published New York, NY : Humana Press, ©2009

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Description 1 online resource (xi, 403 pages) : illustrations Series Springer protocols Methods in molecular biology, 1940-6029 ; 554 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 554. 1064-3745 Contents Biochemical isolation of mtDNA nucleoids from animal cells / D.F. Bogenhagen -- Analysis of mitochondrial DNA by two-dimensional agarose gel electrophoresis / A. Reyes [and others] -- Comparative purification strategies for Drosophila and human mitochondrial DNA replication proteins: DNA polymerase gamma and mitochondrial single-stranded DNA-binding protein / M.T. Oliveira and L.S. Kaguni -- Functional analysis of mutant mitochondrial DNA polymerase proteins involved in human disease / S.S. Chan and W.C. Copeland -- Preparation of human mitochondrial single-stranded DNA-binding protein / M.J. Longley, L.A. Smith and W.C. Copeland -- Methods for assessing binding of mitochondrial transcription factor A (TFAM) to DNA / A. Fukuoh and D. Kang -- Inducible expression in human cells, purification, and in vitro assays for the mitochondrial DNA helicase Twinkle / S. Goffart and H. Spelbrink -- Purification strategy for recombinant forms of the human mitochondrial DNA helicase / T.D. Ziebarth and L.S. Kaguni -- Methods for studying mitochondrial transcription termination with isolated components / P.L. Polosa [and others] -- Oxidative phosphorylation: synthesis of mitochondrially encoded proteins and assembly of individual structural subunits into functional holoenzyme complexes / S.C. Leary and F. Sasarman -- Reactive oxygen species production by mitochondria / A.J. Lambert and M.D. Brand -- Measuring mtDNA damage using a supercoiling-sensitive qPCR approach / S.W. Chan and J.Z. Chen -- Quantitative analysis of oxidized guanine, 8-oxoguanine, in mitochondrial DNA by immunofluorescence method / M. Ohno, S. Oka and Y. Nakabeppu -- In vitro measurement of DNA base excision repair in isolated mitochondria / M.M. Page and J.A. Stuart -- Targeting repair proteins to the mitochondria of mammalian cells through stable transfection, transient transfection, viral transduction, and TAT-mediated protein transduction / C.A. Koczor [and others] -- Construction and characterization of a cell line deficient in repair of mitochondrial, but not nuclear, oxidative DNA damage / S. Oka, M. Ohno and Y. Nakabeppu -- Mitochondrial DNA oxidative damage and mutagenesis in Saccharomyces cerevisiae / L.M. Griffiths [and others] -- Determination of DNA mutation load in human tissues using denaturing HPLC-based heteroduplex analysis / K.S. Lim, R.K. Naviaux and R.H. Haas -- Rapid identification of unknown heteroplasmic mitochondrial DNA mutations with mismatch-specific surveyor nuclease / S. Bannwarth, V. Procaccio and V. Paquis-Flucklinger -- Collection of isolated cells for studying mitochondrial DNA mutations within individual cells / Y. Kraytsberg [and others] -- Quantitative analysis of somatic mitochondrial DNA mutations by single-cell single-molecule PCR / Y. Kraytsberg [and others] -- Measuring DNA precursor pools in mitochondria / C.K. Mathews and L.J. Wheeler -- Establishment of human cell lines lacking mitochondrial DNA / K. Hashiguchi and Q.M. Zhang-Akiyama Summary Annotation Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Here, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA Analysis mitochondriaal dna mitochondrial dna dna mitochondria methodologie methodology mutaties mutations transductie transduction protocollen protocols Molecular Biology (General) Moleculaire biologie (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Mitochondrial DNA -- Laboratory manuals Mitochondrial DNA. Genetics -- Technique. DNA, Mitochondrial Genetic Techniques Genetics -- Technique Mitochondrial DNA Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Stuart, Jeffrey A. (Jeffrey Alan), 1968- ISBN 9781597455213 1597455210

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