| Description |
1 online resource (xxii, 147 pages) : illustrations (some color) |
| Contents |
Part I: Super-Resolution Microscopy and Molecular Imaging Techniques to Probe Biology -- 1. Introduction on Single-Molecule Science -- 2. One Molecule, Two Molecules, Red Molecules, Blue Molecules: Methods for Quantifying Localization Microscopy Data -- 3. Multiscale Fluorescence Imaging -- 4. Long-Read Single-Molecule Optical Maps -- Part II: Protein Folding, Structure, Confirmation, and Dynamics -- 5. Single-Molecule Mechanics of Protein Nanomachines -- 6. Posttranslational Protein Translocation through Membranes at the Single-Molecule Level -- Part III: Mapping DNA Molecules at the Single-Molecule Level -- 7. Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope -- 8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification -- Part IV: Single-Molecule Biology to Study Gene Expression -- 9. Single-Molecule Detection in the Study of Gene Expression |
| Summary |
"Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology"-- Provided by publisher |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Description based on online resource; title from digital title page (viewed on May 14, 2022) |
| Subject |
Molecular biology -- Technique.
|
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Gene mapping -- Technological innovations
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Microscopy -- Technological innovations
|
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Biophysics.
|
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Biophysics
|
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Molecular biology -- Technique
|
| Form |
Electronic book
|
| Author |
Appasani, Krishnarao, 1959- editor.
|
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Appasani, Raghu K., editor
|
| LC no. |
2020041019 |
| ISBN |
9781108525909 |
|
1108525903 |
|
