Title Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols / edited by Bettina Warscheid

Published New York, NY : Humana Press, [2014] ©2014

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Description 1 online resource (xiv, 372 pages) : illustrations (some color) Series Methods in Molecular Biology, 1940-6029 ; Volume No. 1188 Methods in molecular biology (Clifton, N.J.) ; v. 1188. 1064-3745 Contents 1. Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) / Matthias Mann -- 2. Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture / Boumediene Soufi and Boris Macek -- 3. SILAC Labeling of Yeast for the Study of Membrane Protein Complexes / Silke Oeljeklaus, Andreas Schummer, Ida Suppanz, and Bettina Warscheid -- 4. Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei Using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry / Olivera Cirovic and Torsten Ochsenreiter -- 5. Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons / Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert -- 6. SILAC and Alternatives in Studying Cellular Proteomes of Plants / Annemarie Matthes, Karin Köhl, and Waltraud X. Schulze -- 7. In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster / Matthias D. Sury, Jia-Xuan Chen, and Matthias Selbach -- 8. Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse / Soraya Hölper, Aaron Ruhs, and Marcus Krüger -- 9. Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans / Julius Fredens, Kasper Engholm-Keller, Jakob Møller-Jensen, Martin Røssel Larsen, and Nils J. Færgeman -- 10. SILAC-Based Temporal Phosphoproteomics / Chiara Francavilla, Omid Hekmat, Blagoy Blagoev, and Jesper V. Olsen -- 11. Global Ubiquitination Analysis by SILAC in Mammalian Cells / Zhiping Wu, Chan Hyun Na, Haiyan Tan, and Junmin Peng -- 12. Quantifying In Vivo, Site-Specific Changes in Protein Methylation with SILAC / Ho-Tak Lau, Karen A. Lewis, and Shao-En Ong -- 13. Applying SILAC for the Differential Analysis of Protein Complexes / Karsten Boldt, Christian J. Gloeckner, Yves Texier, Felix von Zweydorf, and Marius Ueffing -- 14. Defining Dynamic Protein Interactions Using SILAC-Based Quantitative Mass Spectrometry / Xiaorong Wang and Lan Huang -- 15. Identifying Nuclear Protein-Protein Interactions Using GFP Affinity Purification and SILAC-Based Quantitative Mass Spectrometry / H. Irem Baymaz, Cornelia G. Spruijt, and Michiel Vermeulen -- 16. Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC / Carla Schmidt, Monika Raabe, Reinhard Lührmann, and Henning Urlaub -- 17. Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling / Frederik Sommer, Timo Mühlhaus, Dorothea Hemme, Daniel Veyel, and Michael Schroda -- 18. Protein Correlation Profiling-SILAC to Study Protein- Protein Interactions / Anders R. Kristensen and Leonard J. Foster -- 19. Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC / Andrea C. Becker and Jörn Dengjel -- 20. Design and Application of Super-SILAC for Proteome Quantification / Yair Pozniak and Tamar Geiger -- 21. Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies / Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari, and Tiziana Bonaldi -- 22. Analysis of Secreted Proteins Using SILAC / Jeanette Henningsen, Blagoy Blagoev, and Irina Kratchmarova -- 23. Identification of MicroRNA Targets by Pulsed SILAC / Markus Kaller, Silke Oeljeklaus, Bettina Warscheid, and Heiko Hermeking -- 24. MaxQuant for In-Depth Analysis of Large SILAC Datasets / Stefka Tyanova, Matthias Mann, and Jürgen Cox Summary "Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--Publisher's description Analysis Stable isotope labeling by amino acids in cell culture SILAC eiwitten proteins celkweek cell culture eiwitexpressieanalyse proteomics Micropropagation Microvermeerdering Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed August 6, 2014) Subject Proteomics -- Laboratory manuals Stable isotopes -- Laboratory manuals Amino acids -- Laboratory manuals Cell culture -- Laboratory manuals Mass spectrometry. Amino Acids -- chemistry Isotope Labeling -- methods Cell Culture Techniques Mass Spectrometry mass spectrometry. Proteomics Stable isotopes Amino acids Cell culture Mass spectrometry Biology -- Laboratory manuals Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Warscheid, Bettina, editor ISBN 9781493911424 1493911422

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