| Description |
1 online resource (x, 218 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 486 |
|
Springer protocols |
|
Methods in molecular biology (Clifton, N.J.) ; v. 486
|
|
Springer protocols (Series)
|
| Contents |
Introduction: Cell-based assays for high-throughput screening / W.F. An and N.J. Tolliday -- High-throughput screening of model bacteria / S. Zlitni, J.E. Blanchard and E.D. Brown -- Cell-based assays to probe the ERK MAP kinase pathway in endothelial cells / M.R. Wyler [and others] -- Large-scale small-molecule screen using zebrafish embryos / C.C. Hong -- Whole-animal high-throughput screens: The C. elegans model / E.J. O'Rourke, A.L. Conery and T.I. Moy -- Whole-organism screening: Plants / A. Agee and D. Carter -- Fluorescence-based assays / W.F. An -- Reporter gene assays / A.M.F. Liu [and others] -- Screening for chemical inhibitors of heterologous proteins expressed in yeast using a simple growth-restoration assay / A.D. Balgi and M. Roberge -- Assay for isolation of inhibitors of her2-kinase expression / G. Chiosis and A.B. Keeton -- High-content screening: Flow cytometry analysis / B.S. Edwards [and others] -- High-throughput real-time PCR for detection of gene-expression levels / B.K. Wagner and Z. Arany -- Interpretation of uniform-well readouts / S. Josiah -- Extracting rich information from images / A.E. Carpenter |
| Summary |
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
High throughput screening (Drug development)
|
|
Biological assay.
|
|
Drug Discovery
|
|
Biological Assay
|
|
Drug Evaluation, Preclinical
|
|
Biological assay
|
|
High throughput screening (Drug development)
|
|
Dosage biologique -- Cahiers de laboratoire.
|
|
Évaluation préclinique médicament -- Cahiers de laboratoire.
|
|
High throughput screening
|
|
Untersuchungsmethode
|
|
Zelle
|
|
Biochemistry.
|
|
Biology - General.
|
|
Pharmacy, Therapeutics, & Pharmacology.
|
|
Chemistry.
|
|
Health & Biological Sciences.
|
|
Biology.
|
|
Physical Sciences & Mathematics.
|
|
Pharmacie -- Recherche.
|
|
Essais biologiques.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Clemons, Paul A.
|
|
Tolliday, Nicola J.
|
|
Wagner, Bridget K.
|
| ISBN |
9781603275446 |
|
1603275444 |
|
9781603275453 |
|
1603275452 |
|
