Description |
1 online resource (xxi, 816 pages) : illustrations (some color) |
Series |
Methods in Molecular Biology, 1064-3745 ; 1076 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1076. 1064-3745
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Contents |
Part I: Steady-State Fluorescence Spectroscopy (SSFS). How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra -- Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions -- Quantitative Fluorescence Spectral Analysis of Protein Denaturation -- High-Pressure Fluorescence Applications -- Part II: Time-Resolved Fluorescence Spectroscopy (TRFS). Frequency Domain Fluorometry: Theory and Application -- Polar Plot Representation of Time-Resolved Fluorescence -- Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics -- MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes -- Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins -- Global Analysis of Time-Resolved Fluorescence Data -- Nanometrology -- Upconversion Spectrophotofluorometry -- Subpicosecond Kerr-Gate Spectrofluorometry -- Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay -- Part III: Fluorescent Probe Development (FPD). Biosynthetic Incorporation of Trp Analogs in Proteins -- Optimization of Fluorescent Proteins -- Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family -- Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP). Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope -- Part V: Fluorescence Microscopy: F?rster Resonance Energy Transfer Imaging (FRETim). A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging -- Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM). Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors -- Global Analysis of FRET- FLIM Data in Live Plant Cells -- Time-Resolved Fluorescence Anisotropy Imaging -- Multi-Modal Fluorescence Imaging Spectroscopy -- Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS). Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells -- Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells -- Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS) -- PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching -- Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes -- Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane -- Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS -- Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy -- Brightness Experiments -- Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy -- Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy -- Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS). Single-Molecule Fluorescence of Nucleic Acids -- Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light |
Summary |
Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results |
Analysis |
geneeskunde |
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medicine |
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volksgezondheid |
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public health |
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spectrometrie |
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spectrometry |
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microscopie |
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microscopy |
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afbeelden |
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imagery |
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radiologie |
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radiology |
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biologische technieken |
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biological techniques |
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Medicine (General) |
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Geneeskunde (algemeen) |
Bibliography |
Includes bibliographical references and index |
Notes |
English |
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Online resource; title from PDF title page (SpringerLink, viewed October 14, 2013) |
Subject |
Fluorescence spectroscopy -- Laboratory manuals
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Fluorescence microscopy -- Laboratory manuals
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Fluorimetry.
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Diagnostic imaging.
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Photometry.
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Diagnosis.
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Fluorescence microscopy.
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Methodology.
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Fluorescence spectroscopy.
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Microscopy
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Publication Formats
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Spectrum Analysis
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Fluorometry
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Investigative Techniques
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Diagnostic Imaging
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Chemistry Techniques, Analytical
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Publication Characteristics
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Luminescent Measurements
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Diagnostic Techniques and Procedures
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Photometry
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Diagnosis
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Microscopy, Fluorescence
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Methods
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Spectrometry, Fluorescence
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Laboratory Manuals
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diagnosis.
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fluorescence microscopy.
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methodology.
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Photometry
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Methodology
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Fluorimetry
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Diagnostic imaging
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Diagnosis
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Fluorescence microscopy
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Fluorescence spectroscopy
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Engelborghs, Yves, editor
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Visser, Antonie J. W. G., 1943- editor.
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ISBN |
9781627036498 |
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1627036490 |
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1627036482 |
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9781627036481 |
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